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Our calibration particles are designed for
routine calibration of flow cytometers and are used extensively for QC and
long term performance tracking of flow cytometers. They can also be used
for routine alignment and calibration in fluorescence and confocal
fluorescence microscopy. All calibration particles are supplied in dropper
bottles containing 5 x106 particles.
Rainbow Calibration Particles are a mixture of fluorophores that
allows excitation at any wavelength from 365 to 650nm. It enables
calibration of all channels of the flow cytometer with the same set of
particles of similar size, but with different intensities.
Rainbow Fluorescent Particles are similar to the described above,
except these represent single peak, uniform size particles. The Rainbow
fluorescent particles are usually the brightest peak of the corresponding
Calibration Particles with the exception of P-RFPM-3.0, which has
fluorescent intensity of the stained cells. Since these particles contain
single peak with very small fluorescence and size CV, they are very
useful in the alignment of the optical system of the flow cytometer.
Yellow Calibration Particles are designed for routine calibration
of the fluorescin channel. They contain a set of similar size Yellow
Particles with different fluorescence intensities.
Rainbow Linear Calibration Particles are designed to check the
linearity of the PMT in the linear scale of all available channels. They
are also useful for quantifying the DNA content of cells stained with
Hoechst dye, DAPI, Ethidium bromide, Propidium bromide, YOYO or TOTO.
Allophycocyanin Calibration Particles contain a mixture of 3.0µ
fluorescent particles with different intensity in Allophycocyanin channel
and can be excited with either a He-Ne laser at 632 nm or a diode laser at
635nm.
Blank Calibration Particles are the blank peak of the corresponding
Rainbow Calibration Particles. These particles can be used to set the
threshold of the instrument in each channel.
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